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locus on human chromosome 19 was accomplished by transient expression of AAV Rep functions inside the concentrate on cells . Specific DNA integration is advantageous because it dispels the insertional oncogenesis concerns discussed over.
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Figure six Diagram of the generation and transduction of a self-complementary AAV vector when compared to that of a standard recombinant AAV. Left panel: Based on the AAV DNA replication plan, full-size rAAV genomes of both of those polarities are generated from duplex monomeric (DM) and duplex dimeric (DD) replicative intermediates and separately packaged in AAV capsids. Within the nucleus of transduced cells the single-stranded genomes can both become a target for degradation or be converted into transcriptionally Lively double-stranded templates. The single-to-double strand DNA conversion will depend on complementary chain synthesis or around the recruitment of a complementary genome (i.
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